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Read MoreIdentification of X-chromosomal MAPK regulators by a pooled CRISPR knockout monitor. To comprehensively discover X-encoded MAPK inhibitors, we carried out a chromosome-huge pooled CRISPR knockout display (Fig. 1a). Through transduction of Cas9-expressing mESCs with an X-chromosomal sgRNA expression library, a pool of cells was produced with maximally just one gene mutated for every cell. A…
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