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Identification of X-chromosomal MAPK regulators via a pooled CRISPR knockout display screen. To comprehensively discover X-encoded MAPK inhibitors, we carried out a chromosome-wide pooled CRISPR knockout display screen (Fig. 1a). Through transduction of Cas9-expressing mESCs with an X-chromosomal sgRNA expression library, a pool of cells was produced with maximally a single gene mutated for every…

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Keilhauer EC, Hein MY, Mann M. Accurate protein advanced retrieval by affinity enrichment mass spectrometry (AE-MS) instead than affinity purification mass spectrometry (AP-MS). Kulak NA, Pichler G, Paron I, Nagaraj N, Mann M. Minimal, encapsulated proteomic-sample processing utilized to copy-quantity estimation in eukaryotic cells. Human embryonic stem cells do not alter their X inactivation standing…

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