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Identification of X-chromosomal MAPK regulators by means of a pooled CRISPR knockout monitor. To comprehensively identify X-encoded MAPK inhibitors, we executed a chromosome-large pooled CRISPR knockout screen (Fig. 1a). Through transduction of Cas9-expressing mESCs with an X-chromosomal sgRNA expression library, a pool of cells was created with maximally 1 gene mutated for each mobile. A…

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