No other peptides lowered emission under .60 relative to untreated samples. Anti-FLAG immunblot of lysates from HEK293T cells applied in AKAR4 recordings displaying relative expression concentrations of WT and Δ79-86 FLAG-AKAP79. We also demonstrated that ablating the CaM-binding web site in AKAP79 increases resting PKA phosphorylation in HEK293T cells. CaM N-lobe is not expected for…
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